The Kuwaiti government submitted its resignation on Thursday, State Minister for Cabinet Affairs Rudhan al-Rudhan told state news agency KUNA.
Lawmakers had asked to question three ministers, all members of the ruling al-Sabah family, the latest in a series of challenges by an unusually assertive Arab parliament that have delayed important economic reforms.
"The ministers submitted the resignation to the prime minister, who will refer it to the emir," a parliamentary source told Reuters.
Kuwait's parliament, the most outspoken in a Gulf region has triggered numerous cabinet resignations or reshuffles through questionings.
Sources said on Wednesday they expected the Gulf state's ruling emir, Sheikh Sabah al-Ahmad al-Sabah, to reappoint Prime Minister Sheikh Nasser al-Mohammad al-Sabah to form another cabinet.
While tough questioning of ministers is an everyday occurrence in most parliaments in the world, in Kuwait the questioning is more akin to a direct challenge to the individual and an indirect challenge to the ruler, who has the last say in politics.